Laboratory of Microbial Cultivation and Ecogenomics

Our research focuses on the ecology, evolution, and niche partitioning of freshwater oligotrophs. Small prokaryotes with reduced (streamlined) genomes are the most abundant organisms in the pelagial of freshwater lakes. However, their ecology remains largely elusive because of problems in cultivating environmentally relevant microbes. We apply high-throughput dilution-to-extinction techniques with media mimicking natural conditions to isolate such genome-streamlined oligotrophs and comparative genomics to shed light on their ecological niche, (micro)diversity and evolutionary history.



Group leader

Michaela M. Salcher, PhD

Lab members

Markus Haber, PhD

Cecilia Chiriac, PhD

Hon Lun (Alan) Wong, PhD

Paul Layoun, MSc.

Clafy Fernandes, MSc.

Kostanjšek Fran, MSc.

Henriques Vieira Helena, PhD



We have currently an open position for a postdoc interested in microbial cultivation and ecogenomics! Please check out the announcement at the bottom of the page...



Current projects

EcoFAct – Ecology of abundant freshwater actinobacteria

Financial support: Czech Science Foundation (GACR); Project No.: 20-12496X, Duration: 2020-2024

Principal Investigator: Markus Haber

Team Members: Indranil Mukherjee, Michaela Salcher, Rohit Ghai, Karel Šimek, Vinicius Kavagutti, Cecilia Chiriac

Actinobacteria of the acI lineage (Ca. Nanopelagicales) are ubiquitously present and often among the most abundant microbes in freshwater lakes around the globe. While several hypotheses for their success have been advanced based on culture independent approaches, most remain untested owing to a lack of cultures. Isolation efforts based on the recently reported first successful cultivation of acI strains enabled us to isolate more strains from this group. Here we propose to expand this culture collection and test isolates for physiological and genomic differences related to environmental factors to determine their ecological niches and ecotype diversity. We will address hypotheses pertaining to protection against eukaryotic grazing and phage infection and their interactions with co-occurring auto- and heterotrophic bacteria. Finally, we will examine if their success can be explained by their ability to use light as energy source and its effects on their physiology. Our results will greatly enhance our understanding of the ecological role of this important group.

The proposed research investigates the ecology of acI actinobacteria, one of the most abundant group of freshwater bacteria. Specifically, we will examine niche separation between strains; their interaction with grazer, phages, and sympatric bacteria; and its potential for photoheterotrophy.


Pan-European Lake Sampling - Microbial Eco-genomics (PELAGICS)

Financial support: Czech Science Foundation (GACR); Project No.: 20-12496X, Duration: 2020-2024

Principal Investigator: Rohit Ghai, co-PI: Michaela Salcher

Team Members: Paul Bulzu, Indranil Mukherjee, Alan Wong, Tiberiu Szöke-Nagy, Vojtěch Kasalický, Karel Šimek, Vinicius Kavagutti, Clafy Fernandes, Roudaina Boukheloua, Monika Okrouhlíková, Helena Vieira, Fran Kostanjšek, Petr Macek

Freshwater habitats are critical for all terrestrial life, yet the vast majority of their microbial inhabitants (pro- and eukaryotes) remain enigmatic, outside the bounds of cultivation. The recent development of novel cultivation methods, coupled with advances in sequencing now provides an opportunity to finally unravel freshwater microbial diversity. The PELAGICS project plans a coordinated pan-European sampling campaign (70 lakes) with 24 collaborating scientists from 16 European countries. With novel media mimicking natural conditions and semi-automated high-throughput isolation we aim for stable cultivation and whole-genome sequencing of 500 prokaryotes and 50 unicellular eukaryotes. Moreover, terabyte scale deep-metagenomic sequencing (ca. 15 TB) will allow recovery of thousands of metagenome-assembled genomes for pro-, eukaryotes and viruses. This large-scale effort will finally uncover the microbial diversity (pro- and eukaryotes), their natural interactions and ecological roles in aquatic food webs.

A Pan-European microbial ecology network is proposed to sample 70 lakes towards the goal of stable cultivation, whole-genome sequencing (500 pro- and 50 unicellular eukaryotes) and terabyte-scale deep-metagenomic sequencing to obtain 1000s of genomes of freshwater pro-, eukaryotes and viruses.


Eco-genomics of genome-streamlined freshwater methylotrophs

Financial support: Czech Science Foundation (GACR); Project No.: 19-23469S; Duration: 2019-2021

Principal Investigator: Michaela Salcher

Team Members: Markus Haber, Tanja Shabarova, Paul Bulzu, Paul Layoun, Rohit Ghai, Radka Malá

The most abundant planktonic microbes have reduced genomes and streamlining theory predicts that gene loss is caused by evolutionary selection driven by environmental factors. Yet the evolutionary path of streamlining remains unknown because of obstacles in establishing axenic cultures of such microbes. We developed a targeted isolation technique for abundant genome-streamlined freshwater methylotrophs: ‘Ca. Methylopumilus planktonicus’ (Betaproteobacteria, 1.3 Mbp genome size), that are ideal model organisms for studying microdiversification patterns and the evolution of genome-streamlining per se. The closest relatives of ‘Ca. M. planktonicus’ inhabit lake sediments and the pelagial of oceans, and we propose that the evolutionary origin of the family can be traced back to sediment microbes with medium-sized genomes. Whole genome sequencing of 150 strains and deep sequencing of metagenomes will allow comparative population genomics with the aim to disentangle the underlying ecological reasons for the widespread but yet enigmatic phenomenon of genomestreamlining in aquatic microbes.

The aim of the project is using targeted isolation and whole-genome-sequencing of oligotrophic freshwater ‘Ca. Methylopumilus planktonicus’ (Betaproteobacteria) together with metagenomics to study the evolution of genome-streamlining in planktonic microbes. Identifying microdiversification patterns in closely related taxa.


Eco-genomics of freshwater microbes

Financial support: Money follows Researcher (MFR) grant of the Swiss National Science Foundation (SNSF); Project No.: 310030_185108; Duration: 2019-2020

Principal Investigator: Michaela Salcher

Team Members: Markus Haber



Recent publications (2019-now)

Rosenberg D.R., Haber M., Goldford J., Lalzar M., Aharonovich D., Al-Ashhab A., Lehahn Y., Segrè D., Steindler L., Sher D. (2021) Particle-associated and free-living bacterial communities in an oligotrophic sea are affected by different environmental factors. Environmental Microbiology 23 : 4295-4308. DOI: 10.1111/1462-2920.15611
Piwosz K., Mukherjee I., Salcher M.M., Grujčić V., Šimek K. (2021) CARD-FISH in the sequencing era: Opening a new universe of protistan ecology Frontiers in Microbiology 12 : 640066. DOI: 10.3389/fmicb.2021.640066
Mujakic I., Andrei A., Shabarova T., Kolesár Fecskeová L., Salcher M.M., Piwosz K., Ghai R., Koblížek M. (2021) Common presence of phototrophic Gemmatimonadota in temperate freshwater lakes mSystems 6 : e01241-20.
DOI: 10.1128/mSystems.01241-20
Okazaki Y., Fujinaga S., Salcher M.M., Callieri C., Tanaka A., Kohzu A., Oyagi H., Tamaki H., Nakano S. (2021) Microdiversity and phylogeographic diversification of bacterioplankton in pelagic freshwater systems revealed through long-read amplicon sequencing Microbiome 9 : DOI: 10.1186/s40168-020-00974-y
Shabarova T., Salcher M.M., Porcal P., Znachor P., Nedoma J., Grossart H., Seďa J., Hejzlar J., Šimek K. (2021) Recovery of freshwater microbial communities after extreme rain events is mediated by cyclic succession Nature Microbiology DOI: 10.1038/s41564-020-00852-1
Haber M., Burgsdorf I., Handley K.M., Rubin-Blum M., Steindler L. (2021) Genomic insights into the lifestyles of Thaumarchaeota inside sponges Frontiers in Microbiology 11 : 622824.
DOI: doi.org/10.3389/fmicb.2020.622824
Di Cesare A., Dzhembekova N., Cabello-Yeves P.J., Eckert E.M., Slabakova V., Slabakova N., Peneva E., Bertoni R., Corno G., Salcher M.M., Kamburska L., Bertoni F., Rodriguez-Valera F. , Moncheva S., Callieri C. (2020) Genomic comparison and spatial distribution of different Synechococcus phylotypes in the Black Sea Frontiers in Microbiology 11 : 1979. DOI: doi.org/10.3389/fmicb.2020.01979
Kust A., Řeháková K., Vrba J., Maicher V., Mareš J., Hrouzek P., Chiriac M.C., Benedová Z., Tesařová M., Saurav K. (2020) Insight into unprecedented diversity of cyanopeptides in eutrophic ponds using an MS/MS networking approach Toxins 12 : 561. DOI: doi:10.3390/toxins12090561
Mukherjee I., Salcher M.M., Andrei A., Kavagutti V., Shabarova T., Grujčić V., Haber M., Layoun P., Hodoki Y., Nakano S., Šimek K., Ghai R. (2020) A freshwater radiation of diplonemids Environmental Microbiology 22 : 4658–4668. DOI: doi.org/10.1101/2020.05.14.095992
Piwosz K., Shabarova T., Pernthaler J., Posch T., Šimek K., Porcal P., Salcher M.M. (2020) Bacterial and eukaryotic small-subunit amplicon data do not provide a quantitative picture of microbial communities, but they are reliable in the context of ecological interpretations mSphere 5 : e00052-20. DOI: 10.1128/mSphere.00052-20
Salcher M.M., Andrei A., Bulzu P.A,, Keresztes Z.G., Banciu H.L., Ghai R. (2020) Visualization of Lokiarchaeia and Heimdallarchaeia (Asgardarchaeota) by fluorescence in situ hybridization and catalyzed reporter deposition (CARD-FISH) mSphere 5 : e00686-20. DOI: doi.org/10.1128/mSphere.00686-20
Sizikov S., Burgsdorf I., Handley K.M., Lahyani M., Haber M., Steindler L. (2020), Characterization of sponge‐associated Verrucomicrobia: microcompartment‐based sugar utilization and enhanced toxin–antitoxin modules as features of host‐associated Opitutales. Environmental Microbiology 22: 4669-4688. DOI: https://doi.org/10.1111/1462-2920.15210
Šimek K., Grujčić V., Mukherjee I., Kasalický V., Nedoma J., Posch T., Mehrshad M., Salcher M.M. (2020) Cascading effects in freshwater microbial food webs by predatory Cercozoa, Katablepharidacea and ciliates feeding on aplastidic bacterivorous cryptophytes FEMS Microbiology Ecology 96 : fiaa121. DOI: doi.org/10.1093/femsec/fiaa121
Andrei A., Salcher M.M., Mehrshad M., Rychtecký P., Znachor P., Ghai R. (2019) Niche-directed evolution modulates genome architecture in freshwater Planctomycetes ISME Journal 13 : 1056–1071. DOI: 10.1038/s41396-018-0332-5
Bulzu P.A., Andrei A., Salcher M.M., Mehrshad M., Inoue K. , Kandori H. , Béjà O., Ghai R., Banciu H.L. (2019) Casting light on Asgardarchaeota metabolism in a sunlit microoxic niche Nature Microbiology 4 : 1129–1137. DOI: 10.1038/s41564-019-0404-y
Callieri C., Slabakova V., Dzhembekova N., Slabakova N., Peneva E., Cabello-Yeves P.J., Di Cesare A., Eckert E.M., Bertoni R., Corno G., Salcher M.M., Kamburska L., Bertoni F., Moncheva S. (2019) The mesopelagic anoxic Black Sea as an unexpected habitat for Synechococcus challenges our understanding of global “deep red fluorescence” ISME Journal 13 : 1676-1687. DOI: 10.1038/s41396-019-0378-z
Kavagutti V., Andrei A., Mehrshad M., Salcher M.M., Ghai R. (2019) Phage-centric ecological interactions in aquatic ecosystems revealed through ultradeep metagenomics Microbiome 7 : 135. DOI: 10.1186/s40168-019-0752-0
Salcher M.M., Schaefle D., Kaspar M., Neuenschwander S., Ghai R. (2019) Evolution in action: habitat transition from sediment to the pelagial leads to genome streamlining in Methylophilaceae ISME Journal 13 : 2764–2777. DOI: 10.1038/s41396-019-0471-3

CONTACT

Biology Centre CAS
Institute of Hydrobiology
Na Sádkách 702/7
370 05 České Budějovice

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