Mgr. Vojtěch Kasalický, Ph.D.

Job Position: Postdoc - Department of Aquatic Microbial Ecology (AME)

Contact details

Phone: 420387775887; 420387775883
Room: 26


Interested in the ecology of nonpathogenic freshwater bacteria, their microdiversity, co-existence with other microorganisms and factors explaining their life in the water. Focused to the Limnohabitans genus (Betaproteobacteria) regular members of lakes, reservoirs and (fish)ponds. Isolated more than 50 strains from different habitats. Recently, studying their photosynthetic capability in environmental samples as well as in laboratory cultures. Also, analysing sequenced genomes for the common metabolic traits and differences of the Limnohabitans strains.

Curriculum vitae

Areas of competence:

aquatic microbial ecology, microbial cultivation and isolation, DNA extraction, PCR, sequences alignment, phylogeny, Fluorescence in situ Hybridization, genome sequencing, assembly, annotation and analysis


2006 to 2012: Ph.D. study in Hydrobiology, Faculty of Science, University of South Bohemia, České Budějovice. [Thesis: Ecophysiological characteristics of key members of Betaproteobacteria in freshwater bacterioplankton] Supervisor: Karel Šimek

2003 – 2006: Mgr. (= MSc.) degree in Plant Physiology. Faculty of Biological Sciences, University of South Bohemia, České Budějovice. Thesis: ["Daily rhythms of photosynthetic activity of diatom Thalassiosira weissflogii: impact of natural light-regime and nitrogen limitation] 99 pp.; in Czech. Supervisor: Ondřej Prášil

2000 – 2003: Bc. degree in Biology, Faculty of Biological Sciences, University of South Bohemia, České Budějovice.

Professional experience:

from july 2012: postdoc, Institute of Hydrobiology, Biology Centre CAS

2006 – 2012: Ph.D. student, Institute of Hydrobiology, Biology Centre CAS

2004 – 2006: MSc. student, Laboratory of Photosynthesis, Institute of Microbiology CAS, Třeboň, Czech Republic.

Abroad stays and fellowships:

2004 – 2010: Laboratoire Océanographique de Villefranche sur mer, France; Dr. Markus Weinbauer and Dr. Antoine Sciandra (together three months)

2007 – 2012: Institut für Limnologie OAW, Austria; Dr. Martin Hahn (several short-term stays)

2011: Universität Konstanz, Germany; Dr. Heike Freese (two weeks)

2013: Leibniz Institute of Freshwater Ecology and Inland Fisheries, IGB, Germany; Hans-Peter Grossart, Dr. PD (5 months)

Grant and project participation:

GAČR 17-04828S (2017 – 2019) (PI – Rohit Ghai) Unveiling life strategies of uncultivated viruses in freshwater environments using metagenomics. Member of the research team.

GAČR 15-12197S (2015 – 2017) (PI – Vojtěch Kasalický) Factors regulating the phototrophic activity of freshwater community of Betaproteobacteria.

GAČR 15-09721S (2015 – 2017) (PI – Petr Porcal) The effect of solar light on key members of freshwater Betaproteobacteria. Member of the research team.

GAČR 13-00243S (2013 – 2017) (PI – Karel Šimek) Unveiling life strategies of selected groups of planktonic Betaproteobacteria in relationship to carbon flow to higher trophic levels. Member of the research team.

MŠMT/EE CZ.1.07/2.3.00/30.0032 – (2012-2015) (PI – František Sehnal) Promotion of research [Vytvoření postdoktorandských pozic na Biologickém centru AV ČR k rozvoji biologických disciplín a dosažení globální konkurenceschopnosti.] Postdoc. Member of the research team.

GAČR GAP504/10/0566 (2010 – 2012) (PI – Jan Jezbera) "Distribution, phylogeography and within-taxon ecological differentiation of Limnohabitans cluster and Polynucleobacter necessarius subsp. asymbioticus." Member of the research team.

GAČR GA206/08/0015 (2008 – 2012) (PI – Karel Šimek) "Ecophysiological traits and grazing- and virus-induced mortality of bacterial strains representing major bacterioplankton groups in a reservoir". Member of the research team.


Total found: 20 records
Kasalický V., Zeng Y. H. , Piwosz K., Šimek K., Kratochvilová H., Koblížek M. (2018) Aerobic Anoxygenic Photosynthesis Is Commonly Present within the Genus Limnohabitans Applied and Environmental Microbiology 84 : e02116-17.
DOI: 10.1128/AEM.02116-17
Nguyen N.H.A., Špánek R., Kasalický V., Ribas D., Vlková D., Řeháková H., Kejzlar P., Ševců A. (2018) Different effects of nano-scale and micro-scale zero-valent iron particles on planktonic microorganisms from natural reservoir water Environmental Science: Nano 5 : 1117.
DOI: 10.1039/c7en01120b
Šimek K., Grujčić V., Hahn M., Horňák K., Jezberová J., Kasalický V., Nedoma J., Salcher M., Shabarova T. (2018) Bacterial prey food characteristics modulate community growth response of freshwater bacterivorous flagellates Limnology and Oceanography 63 : 484–502.
DOI: 10.1002/lno.10759
Horňák K., Kasalický V., Šimek K., Grossart H. (2017) Strain-specific consumption and transformation of alga-derived dissolved organic matter by members of the Limnohabitans-C and Polynucleobacter-B clusters of Betaproteobacteria Environmental Microbiology 19 : 4519–4535 .
DOI: 10.1111/1462-2920.13900
Jezberová J., Jezbera J., Znachor P., Nedoma J., Kasalický V., Šimek K. (2017) The Limnohabitans genus harbors generalistic and opportunistic subtypes: evidence from spatiotemporal succession in a canyon-shaped reservoir Applied and Environmental Microbiology 83 : e01530-17.
DOI: 10.1128/AEM.01530-17
Shabarova T., Kasalický V., Šimek K., Nedoma J., Znachor P., Posch T., Pernthaler J., Salcher M. (2017) Distribution and ecological preferences of the freshwater lineage LimA (genus Limnohabitans) revealed by a new double hybridization approach Environmental Microbiology 19 : 1296–1309 .
DOI: 10.1111/1462-2920.13663
Peerakietkhajorn S., Kato Y. , Kasalický V., Matsuura T., Watanabe H. (2016) Betaproteobacteria Limnohabitans strains increase fecundity in the crustacean Daphnia magna: symbiotic relationship between major bacterioplankton and zooplankton in freshwater ecosystem. Environmental Microbiology 18 : 2366–2374..
DOI: 10.1111/1462-2920.12919
Salcher M., Ewert C., Šimek K., Kasalický V., Posch T. (2016) Interspecific competition and protistan grazing affect the coexistence of freshwater betaproteobacterial strains. FEMS Microbiology Ecology 92 : -.
DOI: 10.1093/femsec/fiv156
Grujčić V., Kasalický V., Šimek K. (2015) Prey-specific growth responses of freshwater flagellate communities induced by morphologicallly distinct bacteria from the genus Limnohabitans. Applied and Environmental Microbiology 81 : 4993–5002 .
DOI: 10.1128/AEM.00396-15
Šimek K., Nedoma J., Znachor P., Kasalický V., Jezbera J., Horňák K., Seďa J. (2014) A finely tuned symphony of factors modulates the microbial food web of a freshwater reservoir in spring. Limnology and Oceanography 59 : 1477–1492..
Jezbera J., Jezberová J., Kasalický V., Šimek K., Hahn M. (2013) Patterns of Limnohabitans microdiversity across a large set of freshwater habitats as revealed by reverse line blot hybridization. PLOS ONE 8 : e58527..
DOI: DOI: 10.1371/journal.pone.0058527
Kasalický V., Jezbera J., Hahn M., Šimek K. (2013) The diversity of the Limnohabitans genus, an important group of freshwater bacterioplankton, by characterization of 35 isolated strains. PLOS ONE 8 : e58209..
Šimek K., Kasalický V., Jezbera J., Horňák K., Nedoma J., Hahn M., Bass D., Jost S., Boenigk J. (2013) Differential freshwater flagellate community response to bacterial food quality with a focus on Limnohabitans bacteria. ISME Journal 7 : 1519–1530..
Kasalický V., Jezbera J., Hahn M., Jezberová J., Hejzlar J., Šimek K. (2012) Ecology of planktonic freshwater bacteria of the Limnohabitans genus (Betaproteobacteria). In: Zborník príspevkov XVI. Konferencia SLS a ČLS Od molekúl po ekosystémy, Jasná, June 25–29, 2012, ISBN 978–80–971056–0–0: pp. 59–62.
Straškrábová V., Kasalický V., Šmilauer P., Kopylov A. (2012) Složení mikrobiálního společenstva pelagiálu v komplexním systému Bílého jezera, řeky Šeksny a Rabinské nádrže na horní Volze; význam abiotických a biologických factorů na rozlišení lokalit s využitím ordinační analýzy [Composition of pelagic microbial as In: Zborník príspevkov XVI. Konferencia SLS a ČLS Od molekúl po ekosystémy, Jasná, June 25–29, 2012, ISBN 978–80–971056–0–0: pp. 154–156.
Zeng Y., Kasalický V., Šimek K., Koblížek M. (2012) Genome sequences of two freshwater betaproteobacterial isolates, Limnohabitans species strains Rim28 and Rim47, indicate their capabilities as both photoautotrophs and ammonia oxidizers. Journal of Bacteriology 194 : 6302-6303..
Šimek K., Kasalický V., Zapomělová E., Horňák K. (2011) Alga-derived substrates select for distinct Betaproteobacterial lineages and contribute to niche separation in Limnohabitans strains. Applied and Environmental Microbiology 77 : 7307-7315..
Hahn M., Kasalický V., Jezbera J., Brandt U., Jezberová J., Šimek K. (2010) Limnohabitans curvus gen. nov. sp. nov., a planktonic bacterium isolated from freshwater lake. International Journal of Systematic and Evolutionary Microbiology 60 : 1358–1365..
DOI: DOI: 10.1099/ijs.0.013292-0
Hahn M., Kasalický V., Jezbera J., Brandt U., Šimek K. (2010) Limnohabitans australis sp. nov. isolated from a freshwater pond and emended description of the genus Limnohabitans. International Journal of Systematic and Evolutionary Microbiology 60 : 2946–2950..
Kasalický V., Jezbera J., Šimek K., Hahn M. (2010) Limnohabitans planktonicus sp. nov. and Limnohabitans parvus sp. nov., planktonic betaproteobacteria isolated from a freshwater reservoir, and emended description of the genus Limnohabitans. International Journal of Systematic and Evolutionary Microbiology 60 : 2710–2714..


Biology Centre CAS
Institute of Hydrobiology
Na Sádkách 702/7
370 05 České Budějovice

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